A trial to predict skin irritancy of cosmetic products using cytotoxicity tests

Alternatives to animal testing are being used assess the safety of raw ingredients during process developing cosmetics. However, since cosmetic products composed a variety ingredients, product itself should also be tested. In this study, we attempted evaluate skin irritation potential lotions by modifying existing alternative test methods. To commercial in form an objective indicator, calculated their Irritation Score (IS) based on results searches using keywords related review statements posted Japanese cosmetics and beauty websites. We then modified reconstructed human epidermis (RhE) method lotions. The showed that exposure with higher ISs tended result lower cell viability, many resulted viability. Next, tried vitro short-term (STE) By changing test-substance concentrations times STE method, were able obtain correlated those obtained RhE method. conclusion, both methods helpful for assessing possibility They may useful screening formulations developed as means evaluation before proceeding patch tests.